毛细管电泳法高效筛选8-氧代鸟嘌呤DNA糖基化酶的核酸适配体

8-氧代鸟嘌呤DNA糖基化酶(OGG1)是人体中重要的功能蛋白,在修复DNA氧化性损伤过程中起关键作用。氧化应激等引起的氧化损伤易导致炎症反应的发生,对OGG1的抑制可以一定程度上起到缓解作用;对癌细胞OGG1的抑制有望作为癌症治疗的新方法。目前的研究多集中于小分子对OGG1功能的影响和调控,而OGG1的适配体筛选尚未见报道。作为功能配体,适配体具有合成简单、高亲和力及高特异性等优点。该文筛选了OGG1的核酸适配体,结合毛细管电泳高效快速的优点建立了两种基于毛细管电泳-指数富集进化(CE-SELEX)技术的筛选方法:同步竞争法和多轮筛选法。同步竞争法利用单链结合蛋白(SSB)与核酸库中单链核酸的强结合能力,与目标蛋白OGG1组成竞争体系,并通过增加SSB浓度来增加竞争筛选压力,以去除与OGG1弱结合的核酸序列,一步筛选即可获得与OGG1强结合的核酸序列。多轮筛选法在相同孵育条件和电泳条件下,经3轮筛选获得OGG1的核酸适配体。比较两种筛选方法的筛选结果,筛选结果中频次最高的3条候选核酸适配体序列一致,其解离常数(K_D)值在1.71~2.64 μmol/L之间。分子对接分析结果表明候选适配体1(Apt 1)可能与OGG1中具有修复氧化性损伤功能的活性口袋结合。通过对两种筛选方法的对比,证明同步竞争法更加快速高效,对其他蛋白核酸适配体筛选方法的选择具有一定的指导意义。得到的适配体有望用于OGG1功能调控,以抑制其修复功能。     

最大泡压法测表面张力等温线的数据处理方法比较*

对乙酸、丙酸、正丁酸、2-甲基丁酸、3-甲基丁酸、正戊酸、正己酸、乙醇、丙醇、异丙醇、正丁醇、异丁醇、1,2-丙二醇等13种常用试剂在25 ℃下的表面张力数据拟合方法进行了比较,分析了图解法处理数据及多项式、一阶指数函数、幂函数、Shishkovsky经验公式拟合数据的优缺点。指出Shishkovsky经验公式拟合结果准确,受数据样本量影响小,是理想的表面张力数据拟合方法。     

A Complex Mode Method for Wind-Induced Responses of 6-Parameter Practical Viscoelastic Damping Energy Dissipation Structures Based on the Davenport Wind Speed Spectrum北大核心CSCD

针对六参数实用黏弹性阻尼耗能结构,基于Davenport风速谱系列响应问题进行了系统的研究.首先,利用六参数黏弹性阻尼器的微分型本构关系,建立了耗能结构基于Davenport风速谱激励下的运动方程;然后,运用复模态法将耗能结构的运动方程由二阶微分方程转化为一阶方程,获得了耗能结构系统对风振激励响应的频域解和功率谱密度函数表达式;最后,利用数学恒等式,基于随机振动理论获得了耗能结构系统在Davenport风速谱激励下的响应和阻尼器受力的解析解.该文方法不仅考虑了结构系统在风振激励作用下全振型展开的结果,表达式较现有结果更为简便,效率及精度更高,且适用于非经典阻尼结构.     

跨膜浓度梯度对渗透压囊泡循环活性的调控

本文通过分析囊泡在膨胀状态与孔隙状态的动力学行为,揭示调控膨胀拉伸能与孔隙势能的内在物理参量.建立递推微分方程的分析模型,该模型提供了膨胀-破裂循环特征量膨胀系数在各个循环中初始跨膜浓度梯度依赖性的定量信息.研究得出,通过增加初始跨膜浓度梯度,可加快膨胀系数增长速率(循环数为1时,初始跨膜浓度梯度增加3倍,膨胀系数增长速率增加2.65倍);随着循环数的增多,膨胀系数的增长由线性转变为非线性.此外,初始跨膜浓度梯度与循环次数密切相关,我们的模型计算预测增加初始跨膜浓度梯度可实现循环次数的增多.研究结果为靶组织以可编程方式释放活性生物治疗剂的发展提供了具有实际意义的理论依据.     

氧化铟锡薄膜的磁感应热声分析

基于优良导体在磁场下的涡流效应理论和固体的热声效应理论,建立了氧化铟锡(ITO)导电薄膜磁-热-声效应的理论模型,推导了导电薄膜热致发声的温度振荡和输出声压表达式。对有基底的ITO导电膜进行了磁场下的热声理论计算和实验测试,结果表明：薄膜的温度振荡值随频率呈上升趋势,与电-热-声模型相比趋势相反;薄膜声压的理论值与实验值在频域内的变化趋势基本吻合,验证了理论模型的正确性。进而,根据磁-热-声的理论模型,分析了线圈相关参数对薄膜声压级的影响,结果表明：薄膜声压级随着线圈匝数的增加而增大,随着薄膜与线圈中心距离的增加而减小,随着线圈半径的增加而减小。文中的研究结果拓展了导电薄膜在扬声器等领域的应用。     

Sonoluminescence intensity and ultrasonic cavitation temperature in organic solvents: Effects of generated radicals

Ultrasonic cavitation in organic solvents remains poorly understood in contrast with aqueous systems, largely because of complexities related to solvent decomposition. In this study, we sonicated different types of organic solvents (i.e. linear alkanes, aliphatic alcohols, aromatic alcohols, and acetate esters) under argon saturation. The average temperature of the cavitation bubbles was estimated using the methyl radical recombination method. We also discuss the effects of the physical properties of the solvents, such as vapor pressure and viscosity, on the cavitation temperature. The average cavitation bubble temperature and sonoluminescence intensity were higher in organic solvents with lower vapor pressure; for aromatic alcohols, these values were particularly high. It was found that the specific high sonoluminescence intensities and average cavitation temperatures exhibited in aromatic alcohols are caused by the highly resonance-stable generated radicals. The results obtained in this study are very useful for acceleration of sonochemical reaction in organic solvents, which are indispensable for organic synthesis and material synthesis.     

常压干燥法制备气凝胶的研究进展

气凝胶是一类轻质、低密度的三维纳米多孔固态材料,因其独特的高孔隙率、高比表面积和低导热系数等特性,使其在吸附、催化、保温隔热和隔音等诸多领域具有广泛的用途,目前其相关研究在材料科学领域受到了广泛的关注。气凝胶的制备主要包括溶胶-凝胶过程和湿凝胶干燥两个步骤,湿凝胶的干燥是制备气凝胶过程中至关重要而又较为困难的一步。传统的气凝胶通过超临界干燥制备,工艺复杂、成本高,而且由于干燥过程在高温高压条件下进行,有一定的危险性并且不适宜大规模生产,因此如何通过常压干燥获得高比表面积、高孔隙率、低密度的性能优异的气凝胶是其研究的重要方向之一。本文简要介绍了湿凝胶的制备以及凝胶干燥理论,详细介绍了近年来常压干燥方法气凝胶制备的研究进展,并对其未来发展前景做出了展望。     

Suppression of Liquid-Liquid Phase Separation and Aggregation of Antibodies by Modest Pressure Application

The high colloidal stability of antibody (immunoglobulin) solutions is important for pharmaceutical applications. Inert cosolutes, excipients, are generally used in therapeutic protein formulations to minimize physical instabilities, such as liquid–liquid phase separation (LLPS), aggregation and precipitation, which are often encountered during manufacturing and storage. Despite their widespread use, a detailed understanding of how excipients modulate the specific protein-protein interactions responsible for these instabilities is still lacking. In this work, we demonstrate the high sensitivity to pressure of globulin condensates as a suitable means to suppress LLPS and subsequent aggregation of concentrated antibody solutions. The addition of excipients has only a minor effect. The high pressure sensitivity observed is due to the fact that these flexible Y-shaped molecules create a considerable amount of void volume in the condensed phase, leading to an overall decrease in the volume of the system upon dissociation of the droplet phase by pressure already at a few tens of to hundred bar. Moreover, we show that immunoglobulin molecules themselves are highly resistant to unfolding under pressure, and can even sustain pressures up to about 6 kbar without conformational changes. This implies that immunoglobulins are resistant to the pressure treatment of foods, such as milk, in high-pressure food-processing technologies, thereby preserving their immunological activity.     

气驱油油气混相过程的界面传质特性及其分子机制

油气混相过程的界面传质特性对气驱提高原油采收率技术非常重要。本文针对吉林某油田的实际油组分,采用分子动力学模拟研究了气驱油过程,分析了不同气体和驱替压力下油气两相的状态变化以及界面特性,获得不同驱替气体的最小混相压力(MMP)。结果表明,随着驱替气体压力的升高,气相的密度逐渐增大,油相膨胀密度降低,气相与油相的混合程度增强,油气两相界面厚度增加,界面张力随之减小。同时发现,驱替相中二氧化碳浓度越高,在同等气体压力下,油气界面更厚,油气混合程度更高。纯CO₂驱油得到的MMP远远小于纯N₂驱油,当这两种气体摩尔比为1 : 1混合时MMP介于两种纯气体之间,说明要达到同样的驱油效果二氧化碳需要的压力更小。最后,本文从分子微观作用力角度解释了驱替气体不同时影响油气混相程度的机制,通过分子平均作用势曲线发现油相分子对CO₂的吸引力要大于N₂分子,因此CO₂分子更容易与油相混合,驱替效果更明显。     

ORSIS – News and further developments

The paper deals with the simulation program Off Road Systems Interactive Simulation (ORSIS) which is the w,orldwide leading simulation tool for off road driving of wheeled vehicles. Even though the present state of development allows a very realistic simulation of the man–vehicle–terrain system, there is continuous further improvement in the computer program’s detail. Some of the recent innovations integrated into the program are presented in this paper. These examples were chosen in a way that the scope of the further developments can be demonstrated. They represent three main directions of the work carried out: the refining of the tire–soil-model, the integration of new technologies and subsystems into the vehicle model and the improvement of the man–machine–interface especially in driving simulators.The first part of the paper describes a further development of the tire–soil-model. A significant improvement has been achieved to include the influence of slippery surfaces on traction in combination with the tire tread pattern. Results from finite element method (FEM) as well as real measurements were used to build up an approach, which qualitatively allows the influence of the positive–negative portion of the tire tread and the lug height of the tire tread on traction to be considered. The basic idea is very simple and straightforward. Moreover the calculation costs are very low, so the enhancement does not affect real time operation.In the second part a physical model for the central tire inflation system (CTIS) is presented. With this model it is possible to simulate the complete pneumatic system of a CTIS, including the air compressor with an accumulator, the pressure line and the wheel valves. The components are modelled by their physical parameters, so an adaptation to different existing tire-pressure-control-systems (TPC) can be made. The paper presents a short review of the modelling and a first validation using real measurements. Furthermore the influence of each parameter, e.g. the discharge flow of the compressor on the inflation time, is presented.The third part of the paper describes a further development of the visualization system. The ORSIS OpenGL graphic engine was separated from the main ORSIS simulation and can be run on different PCs controlled via a network. It is therefore possible to build up very cheap multi-channel visualization systems using consumer PCs running under LinuX. The fact that ORSIS itself is running on a normal PC allows the assembly of comparatively cheap driving simulators of a high end simulation quality.